Dynabeads Untouched Human T Cells Kit from Thermo Fisher Scientific

Product Specs
ItemDynabeads Untouched Human T Cells Kit
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog Number11344D
Brand/TrademarkInvitrogen™
QuantityProcesses ~1x10^9 cells total, 100 Isolations
TypeCell Kit
Sample TypePBMC
ReactivityHuman
Cell TypeT Cells (Whole Population)

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Assessing the suitability of cell counting methods during different stages of a cell processing workflow using an ISO 20391-2 guided study design and analysisFront Bioeng BiotechnolAugust 3, 2023Colleen Richards, Sumona Sarkar, Jennifer Kandell, Richard Snyder, Uma Lakshmipathypopulation. In the negative selection method (Dynabeads™ Untouched™ Human T cells Kit, Invitrogen, Cat #11344D), antibodies directed towards CD14, CD16, CD19, CD36, CD56, CD123, and CD235a are added to the cell

(2) Macrophages facilitate tumor cell PD‐L1 expression via an IL‐1β‐centered loop to attenuate immune checkpoint blockadeMedComm (2020)April 1, 2023Cheng Xu, Yu Xia, Bai‐Wei Zhang, Emmanuel Kwateng Drokow, Hua‐Yi Li, Sen Xu, Zhen Wang, Si‐Yuan Wang, Ping Jin, Tian Fang, et al.isolated from healthy donor PBMC via Dynabeads Untouched Human T Cells Kit (Thermo Fisher Scientific, 11344D) according to the instruction, and then activated with anti‐CD3 (5 μg/mL) and anti‐CD28 (5 μg/mL) antibodies

(3) Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12aSTAR ProtocolsJune 17, 2022Xinyu Ling, Liying Chang, Heqi Chen, Tao Liucommercial assays,Critical commercial assays ,, Dynabeads™ Untouched™ Human T Cells Kit,Invitrogen,Cat# 11344D Human IFN gamma Uncoated ELISA kit,Invitrogen,Cat# 88-7316-88 Human TNF alpha Uncoated ELISA kit,Invitrogen,Cat#

(4) CD81 costimulation skews CAR transduction toward naive T cellsProceedings of the National Academy of Sciences of the United States of AmericaFebruary 1, 2022Schultz LM, Czerwinski DK, Levy R, Levy S.pan-T cells were isolated using the Dynabeads Untouched Human T Cell Kit (Thermo Fisher Scientific; 11344D) and isolated naive T cells were purified using the Naive Pan T Cell Isolation Kit (Miltenyi Biotec;

(5) Sustained compensatory p38 MAPK signaling following treatment with MAPK inhibitors induces the immunosuppressive protein CD73 in cancer: combined targeting could improve outcomesMolecular OncologyDecember 1, 2021Mikkel G. Terp, Odd L. Gammelgaard, Henriette Vever, Morten F. Gjerstorff, Henrik J. Ditzelsupernatants were harvested and IFNg secretion was measured using an ELISA kit (Thermo Fisher Scientific, #11344D) according to the manufacturer’s guidelines. 2.9 In vivo studies Murine 4T1 cells (1 x 105) were

(6) Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarctioneLifeJuly 22, 2021Daniela Fraccarollo, Jonas Neuser, Julian Möller, Christian Riehle, Paolo Galuppo, Johann Bauersachsof monocytes Isolation of CD3+ T-cells was performed using Dynabeads Untouched Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571; Invitrogen)

(7) Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patientsNature communicationsFebruary 18, 2021Xydia M, Rahbari R, Ruggiero E, Macaulay I, Tarabichi M, Lohmayer R, Wilkening S, Michels T, Brown D, Vanuytven S, et al.contaminating cells using the Dynal T cell Negative Isolation Kit or the Dynabeads Untouched Human T Cell Kit (11344D, Dynal, Invitrogen, 1:6). PBMC-derived adherent cells were used to differentiate monocytes into mature

(8) Double-Edged Sword: Interleukin-2 Promotes T Regulatory Cell Differentiation but Also Expands Interleukin-13- and Interferon-γ-Producing CD8⁺ T Cells via STAT6-GATA-3 Axis in Systemic Lupus ErythematosusFrontiers in immunologyJanuary 1, 2021Kato H, Perl A.isolated by negative selection using untouched human T cell isolation kit (Life technologies, Cat# 11344D). Purity of CD3+ T cells was conﬁrmed to be above 97%. Cells were cultured in RPMI culture media with

(9) Monocyte TREM-1 Levels Associate With Anti-TNF Responsiveness in IBD Through Autophagy and Fcγ-Receptor Signaling PathwaysFrontiers in immunologyJanuary 1, 2021Prins MM, Verstockt B, Ferrante M, Vermeire S, Wildenberg ME, Koelink PJ.isolated from PBMCs of a different donor by negative isolation (Dynabeads Untouched Human T Cells #11344D, Invitrogen). After 2 days the full monoclonal anti-TNF adalimumab (Humira®, AbbVie, Wavre, Belgium)

(10) CAMK1D Triggers Immune Resistance of Human Tumor Cells Refractory to Anti-PD-L1 TreatmentCancer immunology researchSeptember 1, 2020Volpin V, Michels T, Sorrentino A, Menevse AN, Knoll G, Ditz M, Milenkovic VM, Chen CY, Rathinasamy A, Griewank K, et al.fraction of CD138-sorted bone marrow 155 cells using Untouched Human T cells Dynabeads (Invitrogen #11344D) following manufacturer’s 156 instructions. Cells were stained for anti-CD3 (Pacific Blue™ anti-human

(11) A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis BHuman vaccines & immunotherapeuticsApril 2, 2020Ma A, Motyka B, Gutfreund K, Shi YE, George R.by negative selection using Dynal Biotech T cell negative selection kit (Thermo Fisher Scientific, 11344D) as per manufacturer’s procedure. Exceptions were that matched plasma was used instead of BSA and FBS.

(12) B7-H3 specific T cells with chimeric antigen receptor and decoy PD-1 receptors eradicate established solid human tumors in mouse modelsOncoimmunologyJanuary 1, 2020Huang B, Luo L, Wang J, He B, Feng R, Xian N, Zhang Q, Chen L, Huang G.cells (PBMCs) from healthy donors were negatively purified using a pan T-cell isolation kit (Invitrogen,11344D), and then activated by culture with anti-CD3/CD28 beads (Thermo Fisher Scientific,11131D) according

(13) Genome-scale CRISPR activation screen uncovers tumor-intrinsic modulators of CD3 bispecific antibody efficacyScientific ReportsDecember 1, 2019Corinne E. Decker, Tara Young, Elizabeth Pasnikowski, Joyce Chiu, Hang Song, Yi Wei, Gavin Thurston, Christopher Dalyisolated by negative selection using the Dynabeads Untouched Human T cells kit (Invitrogen, catalog number 11344D). Freshly-isolated T cells were co-cultured with human tumor cell lines at indicated effector:target

(14) Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5BMC biotechnologyApril 24, 2019Lv Y, Xiao FJ, Wang Y, Zou XH, Wang H, Wang HY, Wang LS, Lu ZZ.isolated from MNCs by using a T cell negative isolation kit (Dynabeads Untouched Human T Cells Kit, CAT#11344D; Life Technologies). Isolated T cells were cultured in X-VIVO 15 medium (CAT#04-418Q; Lonza) supplemented

(15) Global alteration of T-lymphocyte metabolism by PD-L1 checkpoint involves a block of de novo nucleoside phosphate synthesisCell discoveryJanuary 1, 2019Palaskas NJ, Garcia JD, Shirazi R, Shin DS, Puig-Saus C, Braas D, Ribas A, Graeber TG.previously expanded for 7 days were isolated by immunomagnetic negative enrichment kit (ThermoFisher #11344D) and seeded in triplicate wells at 8.4 × 105cells in 1.2 mL glucose-free RPMI (ThermoFisher #11879020)

(16) PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patientsOncotargetAugust 10, 2018Sponaas AM, Yang R, Rustad EH, Standal T, Thoresen AS, Dao Vo C, Waage A, Slørdahl TS, Børset M, Sundan A.cells were enriched to > 50% purity from the non-adherent population by negative selection (Invitrogen 11344D). CD3+PD1+ T cells were further enriched using biotinylated anti-PD1 (BioLegend 329934) and streptavidin

(17) Glyco-Engineered Anti-Human Programmed Death-Ligand 1 Antibody Mediates Stronger CD8 T Cell Activation Than Its Normal Glycosylated and Non-Glycosylated CounterpartsFrontiers in immunologyJanuary 1, 2018Goletz C, Lischke T, Harnack U, Schiele P, Danielczyk A, Rühmann J, Goletz S.#11350D or Miltenyi Biotec, #130-096-537), B cells (Invitrogen, #11351D), and T cells (Invitrogen, #11344D). Cytotoxicity Assays The ADCC assay against DU-145 was performed as europium release assay using

(18) Conformational Variants of the Individual HLA-I Antigens on Luminex Single Antigen Beads Used in Monitoring HLA Antibodies: Problems and SolutionsTransplantationApril 1, 2017Vadim Jucaud, Mepur H. Ravindranath, Paul I. Terasakiapproval. T cells were isolated using Dynabeads (Invitrogen by Life Technologies, Carlsbad, CA, Cat 11344D), following manufacturer's negative selection protocol. The purified T cells were aliquoted (2 105

(19) Functionality and opposite roles of two interleukin 4 haplotypes in immune cellsGenes & ImmunityJanuary 5, 2017G Anovazzi, M C Medeiros, S C Pigossi, L S Finoti, T M Souza Moreira, M P A Mayer, C F Zanelli, S R Valentini, C Rossa-Junior, R M Scarel-Caminagasorting (Dynabeads untouched human monocytes kit, #11350D; Dynabeads untouched human T cells kit, #11344D, Invitrogen). After separation, all cells were cultured overnight in RPMI 1640 supplemented with 1%

(20) Increased Mitochondrial Electron Transport Chain Activity at Complex I Is Regulated by N-Acetylcysteine in Lymphocytes of Patients with Systemic Lupus ErythematosusAntioxidants & Redox SignalingJuly 1, 2014Edward Doherty, Zachary Oaks, Andras Perlwww.liebertpub.com/ars cells were isolated by negative selection using untouched human T-cell isolation kit (Catalogue No. 11344D; Life Technologies, Carlsbad, CA). Their purity was confirmed upon each isolation to be ‡ 97% (data

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Dynabeads Untouched Human T Cells Kit from Thermo Fisher Scientific

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Dynabeads Untouched Human T Cells Kit from Thermo Fisher Scientific

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